Data from: Structural instability and divergence from conserved residues underlie intracellular retention of mammalian odorant receptors

Public

  • Mammalian odorant receptors are a diverse and rapidly evolving set of G protein-coupled receptors expressed in olfactory cilia membranes. Most odorant receptors show little to no cell surface expression in non-olfactory cells due to endoplasmic reticulum retention, which has slowed down biochemical studies. Here, we provide evidence that structural instability and divergence from conserved residues of individual odorant receptors underlie intracellular retention using a combination of large-scale screening of odorant receptors cell surface expression in heterologous cells, point mutations, structural modeling, and machine learning techniques. We demonstrate the importance of conserved residues by synthesizing "consensus" odorant receptors that show high levels of cell surface expression similar to conventional G protein-coupled receptors. Furthermore, we associate in silico structural instability with poor cell surface expression using molecular dynamics simulations. We propose an enhanced evolutionary capacitance of olfactory sensory neurons that enable the functional expression of odorant receptors with cryptic mutations. ... [Read More]

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18 files (3.94 MB)
Data Citation
  • Ikegami, K., de March, C. A., Nagai, M. H., Ghosh, S., Do, M., Sharma, R., ... Matsunami, H. (2019). Data from: Structural instability and divergence from conserved residues underlie intracellular retention of mammalian odorant receptors. Duke Digital Repository. https://doi.org/10.7924/r40867k2k
DOI
  • 10.7924/r40867k2k
Publication Date
ARK
  • ark:/87924/r40867k2k
Collection Dates
  • 12/9/2019
Type
Format
Funding Agency
  • NIH
Grant Number
  • R01-GM097261
  • DC014423
  • DC016224
Contact
Title
  • Data from: Structural instability and divergence from conserved residues underlie intracellular retention of mammalian odorant receptors

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