Data from: The control of colonic motility using electrical stimulation to modulate enteric neural circuitry

Barth, Bradley; Grill, Warren M.; Spencer, Nick; Travis, Lee

Data from: The control of colonic motility using electrical stimulation to modulate enteric neural circuitry

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Electrical stimulation of the enteric nervous system (ENS) is an attractive approach to modify gastrointestinal transit. Colonic motor complexes (CMCs) occur with a periodic rhythm, but the ability to elicit a premature CMC depends, at least in part, upon the intrinsic refractory properties of the ENS, which are presently unknown. The objectives of this study were to record myoelectric complexes (MCs, the electrical correlates of CMCs) in the smooth muscle and (i) determine the refractory periods of MCs, (ii) inform and evaluate closed-loop stimulation to repetitively evoke MCs, and (iii) identify stimulation methods to suppress MC propagation. We dissected the colon from male and female C57BL/6 mice, preserving the integrity of intrinsic circuitry while removing the extrinsic nerves, and measured properties of spontaneous and evoked MCs in vitro. Hexamethonium abolished spontaneous and evoked MCs, confirming the necessary involvement of the ENS for electrically-evoked MCs. Electrical stimulation reduced the mean interval between evoked and spontaneous CMCs (24.6 ± 3.5 vs 70.6 ± 15.7 s, p = 0.0002, n = 7). The absolute refractory period was 4.3 s (95% CI = 2.8 - 5.7 s, R2 = 0.7315, n = 8). Electrical stimulation lead to arrest of fluid distention-evoked propagating MC, and following cessation of stimulation propagation resumed at an increased velocity (n = 9). The timing parameters of electrical stimulation increased the rate of evoked MCs, including the duration of entrained MCs, and provide insights into timing considerations for designing neuromodulation strategies to treat colonic dysmotility. ... [Read More]

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Data Citation

Barth, B., Grill, W., Spencer, N., & Travis, L. (2020). Data from: The control of colonic motility using electrical stimulation to modulate enteric neural circuitry. Duke Research Data Repository. https://doi.org/10.7924/r4bk1dq8n

Creator: Barth, Bradley
Grill, Warren M.
Spencer, Nick
Travis, Lee
DOI: 10.7924/r4bk1dq8n
Subject: Colonic motor complex
Enteric nervous system
Closed-loop controller
Neurogastroenterology
Neuromodulation
Publication Date: October 6, 2020
ARK: ark:/87924/r4bk1dq8n
Affiliation: Biomedical Engineering
Visceral Neurophysiology Laboratory, College of Medicine and Public Health, Centre for Neuroscience, Flinders University, Bedford Park, South Australia, 5042, Australia
Publisher: Duke Research Data Repository
Type: Dataset
Format: TXT
CSV
Related Materials: Barth, B., Grill, W. M., Spencer, N. J., & Travis, L. (2021). "Control of colonic motility using electrical stimulation to modulate enteric neural activity". American Journal of Physiology. https://doi.org/10.1152/ajpgi.00463.2020
Contact: Bradley Barth; bradley.barth@duke.edu, ORCID 0000-0002-4344-7182
License: Creative Commons BY Attribution 4.0 International
Title: Data from: The control of colonic motility using electrical stimulation to modulate enteric neural circuitry
License: https://creativecommons.org/licenses/by/4.0/
Repository: Duke Research Data Repository

Title	Date Uploaded	Visibility	Actions
BARTH_CMC_README.txt	2021-02-18	Public	Download
2b_MC_interval.csv	2020-10-06	Public	Download
2c_median_MC_interval.csv	2020-10-06	Public	Download
3c_refractory_period_at_1T.csv	2020-10-06	Public	Download
3d_relative_refractory_period.csv	2020-10-06	Public	Download
3e_absolute_refractory_period.csv	2020-10-06	Public	Download
4_entrainment.csv	2020-10-06	Public	Download
8f_propagation_velocity.csv	2020-10-06	Public	Download
supplementary_fig_1.pdf	2021-02-18	Public	Download
supplementary_fig_2.pdf	2021-02-18	Public	Download

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