• Electrical stimulation of the enteric nervous system (ENS) is an attractive approach to modify gastrointestinal transit. Colonic motor complexes (CMCs) occur with a periodic rhythm, but the ability to elicit a premature CMC depends, at least in part, upon the intrinsic refractory properties of the ENS, which are presently unknown. The objectives of this study were to record myoelectric complexes (MCs, the electrical correlates of CMCs) in the smooth muscle and (i) determine the refractory periods of MCs, (ii) inform and evaluate closed-loop stimulation to repetitively evoke MCs, and (iii) identify stimulation methods to suppress MC propagation. We dissected the colon from male and female C57BL/6 mice, preserving the integrity of intrinsic circuitry while removing the extrinsic nerves, and measured properties of spontaneous and evoked MCs in vitro. Hexamethonium abolished spontaneous and evoked MCs, confirming the necessary involvement of the ENS for electrically-evoked MCs. Electrical stimulation reduced the mean interval between evoked and spontaneous CMCs (24.6 ± 3.5 vs 70.6 ± 15.7 s, p = 0.0002, n = 7). The absolute refractory period was 4.3 s (95% CI = 2.8 - 5.7 s, R2 = 0.7315, n = 8). Electrical stimulation lead to arrest of fluid distention-evoked propagating MC, and following cessation of stimulation propagation resumed at an increased velocity (n = 9). The timing parameters of electrical stimulation increased the rate of evoked MCs, including the duration of entrained MCs, and provide insights into timing considerations for designing neuromodulation strategies to treat colonic dysmotility. ... [Read More]

Total Size

10 files (2.08 MB)

Data Citation

• Barth, B., Grill, W., Spencer, N., & Travis, L. (2020). Data from: The control of colonic motility using electrical stimulation to modulate enteric neural circuitry. Duke Research Data Repository. https://doi.org/10.7924/r4bk1dq8n

Creator

• Travis, Lee
• Barth, Bradley
• Spencer, Nick
• Grill, Warren

DOI

• 10.7924/r4bk1dq8n

Subject

• Enteric nervous system
• Neuromodulation
• Closed-loop controller
• Neurogastroenterology
• Colonic motor complex

Publication Date

• October 6, 2020

ARK

• ark:/87924/r4bk1dq8n

Affiliation

• Visceral Neurophysiology Laboratory, College of Medicine and Public Health, Centre for Neuroscience, Flinders University, Bedford Park, South Australia, 5042, Australia
• Biomedical Engineering

Publisher

• Duke Research Data Repository

Type

• Dataset

Format

• TXT
• CSV

Related Materials

• Barth, B., Grill, W. M., Spencer, N. J., & Travis, L. (2021). "Control of colonic motility using electrical stimulation to modulate enteric neural activity". American Journal of Physiology.  https://doi.org/10.1152/ajpgi.00463.2020

Contact

• Bradley Barth; bradley.barth@duke.edu, ORCID 0000-0002-4344-7182

Rights

• Creative Commons BY Attribution 4.0 International

Title

• Data from: The control of colonic motility using electrical stimulation to modulate enteric neural circuitry

Rights

• https://creativecommons.org/licenses/by/4.0/

Repository

• Duke Research Data Repository

Items

Thumbnail	Title	Date Uploaded		Actions
	BARTH_CMC_README.txt	2021-02-18	Public	Download
	2b_MC_interval.csv	2020-10-06	Public	Download
	2c_median_MC_interval.csv	2020-10-06	Public	Download
	3c_refractory_period_at_1T.csv	2020-10-06	Public	Download
	3d_relative_refractory_period.csv	2020-10-06	Public	Download
	3e_absolute_refractory_period.csv	2020-10-06	Public	Download
	4_entrainment.csv	2020-10-06	Public	Download
	8f_propagation_velocity.csv	2020-10-06	Public	Download
	supplementary_fig_1.pdf	2021-02-18	Public	Download
	supplementary_fig_2.pdf	2021-02-18	Public	Download