Data from: Engineering the microstructure and spatial bioactivity of MAP scaffolds instructs vasculogenesis in vitro and translates to vessel formation in vivo

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  • This data was collected at Duke University in the Segura Lab. The goal of these studies was to explore the impact of microgel heterogeneity (microstructure, spatial bioactivity) on vasculogensis both in vitro and in vivo.
    In tissues where the vasculature is either lacking or abnormal, biomaterials can be designed to promote vessel formation and enhance tissue repair. In this work, we independently tune the microstructure and bioactivity of microporous annealed particle (MAP) scaffolds to guide cell growth in 3D and promote de novo assembly of endothelial progenitor-like cells into vessels. We implement both in silico characterization and in vitro experimentation to elucidate an optimal scaffold formulation for vasculogenesis. We determine that MAP scaffolds with pore volumes on the same order of magnitude as cells facilitate cell growth and vacuole formation. We achieve spatial control over cell spreading by incorporating adhesive microgels in well-mixed, heterogeneous MAP scaffolds. While we demonstrate that integrin engagement is the primary driver of network formation in these materials, introducing adhesive microgels loaded with heparin nanoparticles leads to the formation of vascular tubes after 3 days in culture. We then show in vivo that this unique scaffold formulation enhances vessel maturation in a wound healing model and instructs differential vascular development in the tumor microenvironment. Taken together, this work determines the optimal microstructure and ligand presentation within MAP scaffolds that leads to vascular constructs in vitro and facilitates vessel formation in vivo.
    The details of the motivation, methods, analysis, and results can be found in our publication (DOI 10.1002/adfm.202400567)     ... [Read More]

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9 files (65.7 GB)
Data Citation
  • Anderson, A. R., Caston, E. L. P., Riley, L., Nguyen, L., Ntekoumes, D., Gerecht, S., & Segura, T. (2024). Data from: Engineering the microstructure and spatial bioactivity of MAP scaffolds instructs vasculogenesis in vitro and translates to vessel formation in vivo. Duke Research Data Repository. https://doi.org/10.7924/r4wm1hb9r
DOI
  • 10.7924/r4wm1hb9r
Publication Date
ARK
  • ark:/87924/r4wm1hb9r
Collection Dates
  • 2022-2023
Language
Type
Related Materials
  • Anderson, A. R., Caston, E. L. P., Riley, L., Nguyen, L., Ntekoumes, D., Gerecht, S., & Segura, T. (2024). Engineering the microstructure and spatial bioactivity of MAP scaffolds instructs vasculogenesis in vitro and translates to vessel formation in vivo. In press
Funding Agency
  • National Institutes of Health
Grant Number
  • R01NS112940
Title
  • Data from: Engineering the microstructure and spatial bioactivity of MAP scaffolds instructs vasculogenesis in vitro and translates to vessel formation in vivo
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Thumbnail Title Date Uploaded Visibility Actions
file details Figure 2 - ugel size.zip 2024-12-03 Public Download
file details Figure 3 - ugel mixing.zip 2024-12-03 Public Download
file details Figure 4 - het MAP paths.zip 2024-12-03 Public
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file details Figure 5 - het MAP day 1.zip 2024-12-03 Public
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file details Figure 6 - het MAP day 3.zip 2024-12-03 Public
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file details Figure 7 - wound healing.zip 2024-12-03 Public Download
file details Figure 8 - tumor vasc.zip 2024-12-03 Public Download
file details Readme_VascMAP.txt 2024-12-03 Public Download
file details Supplemental Figures.zip 2024-12-03 Public Download

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