- 3D single-particle tracking has the potential to resolve the molecular-level forces that dictate particle motion in biological systems. However, the information gleaned from 3D single-particle tracking often cannot resolve underlying nanoscale potentials due to limited spatiotemporal resolution. To this end, we introduce an active-feedback 3D tracking microscope that utilizes silver nanoparticles (AgNPs) as probes to study intricate biophysical events in live cells at the nanometer and microsecond scales. Due to the extremely high and durable scattering photon flux of the plasmonic particles, a 1 MHz sampling frequency at nanometer precision in all three dimensions can be achieved over an unlimited observation duration. In this work, we ... [Read More]
- Total Size
- 22 files (2.58 GB)
- Data Citation
- Hou, S., Zhang, C., Niver, A. & Welsher, K. (2022). Data from: Mapping nanoscale forces and potentials in live cells with microsecond 3D single-particle tracking. Duke Research Data Repository. https://doi.org/10.7924/r4dv1q87f
- DOI
- 10.7924/r4dv1q87f
- Publication Date
- December 9, 2022
- ARK
- ark:/87924/r4dv1q87f
- Affiliation
- Publisher
- Type
- Related Materials
- Funding Agency
- NIH
- Grant Number
- R35GM124868
- Contact
- Kevin Welsher, 919-660-1509, kevin.welsher@duke.edu, https://orcid.org/0000-0002-3180-279X
- Title
- Data from: Mapping nanoscale forces and potentials in live cells with microsecond 3D single-particle tracking
- Repository
Thumbnail | Title | Date Uploaded | Visibility | Actions |
---|---|---|---|---|
readme.txt | 2022-12-09 | Download | ||
AOI data.mat | 2022-12-09 | Download | ||
3DSMARTER-github.zip | 2022-12-09 | Download | ||
12 seg data final sorted.mat | 2022-12-09 | Download | ||
200304 TR003 filopodia 1usec 70_120s.mat | 2022-12-09 | Download | ||
200304 TR003 filopodia 1usec 400_460s.mat | 2022-12-09 | Download | ||
200304 TR003 raw tracking data 590_650s.mat | 2022-12-09 | Download | ||
cyl data 70-120s.mat | 2022-12-09 | Download | ||
cyl data 150-200s.mat | 2022-12-09 | Download | ||
cyl data 245-310s.mat | 2022-12-09 | Download |