This repository contains data related to: 

Mapping nanoscale forces and potentials in live cells with microsecond 3D single-particle tracking
Shangguo Hou, Chen Zhang, Anastasia Niver, Kevin Welsher
bioRxiv 2022.06.27.497788; doi: https://doi.org/10.1101/2022.06.27.497788

MATLAB code to analyze the data can be found at:
https://github.com/welsherlab/3DSMARTER
A zip of file of the code is included in this repository: 3DSMARTER-github.zip

Notes on .mat files:
200304 TR003 raw tracking data 590_650s.mat
Contains raw tracking data binned at 1 usec intervals. Variables include:
ktPos – xy laser coordinates TAGPos – z laser coordinate n - # of photons xStage – X piezo readout yStage – Y piezo readout zStage – Z piezo readout. Should be loaded before call to bayesProcessing.m 

cyl data 70-120s.mat ~ cyl data 900-960s.mat
These .mat files store fitting parameters that can be directly obtained through the script cyl_fit_70_120s.m. The stored data underline the edges of top and bottom surface of the cylinder found. One can easily obtain the center of each face and then build the center axis of the cylinder. 

200304 TR003 filopodia 1usec 70_120s.mat
200304 TR003 filopodia 1usec 400_460s.mat
These .mat files store MHz Bayesian reconstructed coordinates in the specified time intervals. With corresponding cylinder fit parameters, one can convert Cartesian coordinates to cylinder coordinates.

12 seg data final sorted.mat
This .mat file stores a structured variable that keeps the cylinder parameters of 13 segments of 50-60 second duration. Data are sorted according to ascending order of start time. User can find other useful information like the radius of each cylinder fit.

AOI data.mat
This file stores a structured variable that records the raw data from manually labelled areas of interest (AOIs). There are a total of 130 ‘hot spots’ from 13 segments.

res time data final sorted.mat
This file contains the same data as above file except that indices of spots inside each AOI are removed for faster importing. 

unwrapped 2d traj 590-650s.mat
This file contains 2-dimensional cylindrical data where 3D spots from Cartesian system are converted to cylindrical data, unwrapped and projected onto cylindrical surface with a radius of average radius of all spots. Data unwrapping was necessary to visualize 2D diffusion as theta are represented as -180 to 180 degrees and show discontinuity at the edges.