Data from: Gene delivery from granular scaffolds for tunable biologics manufacturing

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The following dataset corresponds to all raw data and figures for the manuscript: E. Kurt, G. Devlin, A. Asokan, T. Segura, Gene Delivery From Granular Scaffolds for Tunable Biologics Manufacturing. Small 2024, 20, 2309911. https://doi.org/10.1002/smll.202309911

Our understanding of the molecular basis for disease has generated a myriad of therapeutic biologics, including therapeutic proteins, antibodies, and viruses. However, the promise that biologics can resolve currently incurable diseases hinges in their manufacturability. These therapeutics require that their genetic material be introduced to mammalian cells such that the cell machinery can manufacture the biological components. These are then purified, validated, and packaged. Most manufacturing uses batch processes that collect the biologic a few days following genetic modification, due to toxicity or difficulty in separating product from cells in a continuous operation, limiting the amount of biologic that can be produced and resulting in yearlong backlogs. Here, a scaffold-based approach for continuous biologic manufacturing is presented, with sustained production of active antibodies and viruses for 30 days. The use of a scaffold-based biologic production enabled perfusion-based bioreactors to be used, which can be incorporated into a fully continuous process.
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Data Citation

Kurt, E., Devlin, G., Asokan, A., & Segura, T. (2024). Data from: Gene delivery from granular scaffolds for tunable biologics manufacturing. Duke Research Data Repository. https://doi.org/10.7924/r4280h775

Creator: Asokan, Aravind
Devlin, Garth
Segura, Tatiana
Kurt, Evan
DOI: 10.7924/r4280h775
Subject: Cell culture
CHO
Biologics
Biomaterials
Hydrogels
Nanoparticles
Adeno-associated virus (AAV)
HEK293
Antibodies
Publication Date: August 28, 2024
ARK: ark:/87924/r4280h775
Affiliation: Biomedical Engineering
Publisher: Duke Research Data Repository
Type: Dataset
Format: PDF
MP4
TXT
DOCX
TIFF
PZFX
XLSX
CSV
Related Materials: E. Kurt, G. Devlin, A. Asokan, T. Segura, Gene Delivery From Granular Scaffolds for Tunable Biologics Manufacturing. Small 2024, 20, 2309911. https://doi.org/10.1002/smll.202309911
Funding Agency: National Institutes of Health
National Institute of General Medical Sciences
Grant Number: T32GM008555
R01NS094599
Contact: Tatiana Segura, Ph.D.: tatiana.segura@duke.edu, https://orcid.org/0000-0003-1569-8686
License: Creative Commons BY-NC Attribution-NonCommercial 4.0 International
Title: Data from: Gene delivery from granular scaffolds for tunable biologics manufacturing
License: https://creativecommons.org/licenses/by-nc/4.0/
Repository: Duke Research Data Repository

Title	Date Uploaded	Visibility	Actions
Figure 1 - Single and Dual Plasmid Applications.zip	2024-08-28	Public	Download
Figure 2 - Triple plasmids for AAV in static FLIP.zip	2024-08-28	Public	Download
Figure 3 - AAV in suspension scaffolds.zip	2024-08-28	Public	Download
Figure 4 - Suspension FLIP scaffold optimization.zip	2024-08-28	Public	Download
Figure 5 - Perfusion scaffold system.zip	2024-08-28	Public	Download
Figure 6 - Normalized side-by-side comparison.zip	2024-08-28	Public	Download
Figure S1 - Scaffold loading and properties.zip	2024-08-28	Public	Download
Figure S2 - Static AAV supporting data.zip	2024-08-28	Public	Download
Figure S3 - Suspension scaffold supporting.zip	2024-08-28	Public	Download
Figure S4 - Suspension AAV supporting.zip	2024-08-28	Public	Download

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