This study aimed to develop and validate a robust analytical method to quantify and screen for environmental contaminants in human placenta tissue using QuEChERS-based extraction that was optimized for high recovery and reproducibility while minimizing matrix interference. Analysis was performed using gas chromatography–high resolution mass spectrometry (GC-HRMS) with isotope dilution for targeted quantitation of 119 semi-volatile organic compounds (SVOCs). The validated method was applied to 50 placenta samples collected from a North Carolina pregnancy cohort (Project HOPE 1000).
