Data from: Inhibition of mitochondrial complex III causes dopaminergic neurodegeneration by redox stress in Caenorhabditis elegans

Environmental factors including chemical exposures are important contributors to Parkinson's disease (PD). Nearly all well-validated chemicals involved in PD affect mitochondria and the great majority of those identified inhibit mitochondrial complex I causing ATP depletion and oxidative stress. We hypothesized that inhibition of mitochondrial complex III would also cause dopaminergic neurodegeneration. Using Caenorhabditis elegans to evaluate the in vivo effects of the complex III-inhibiting pesticides antimycin A and pyraclostrobin we found that both caused neurodegeneration. Neurodegeneration was specific to the dopamine neurons and complex III inhibition caused a more-oxidized cellular environment in those neurons. Pharmacological and genetic antioxidant interventions rescued neurodegeneration but energetic rescue attempts did not. Finally optogenetic production of superoxide anion specifically at complex III caused dopaminergic neurodegeneration. Thus redox stress at complex III is sufficient for dopaminergic neurodegeneration and redox stress following chemical inhibition is necessary for dopaminergic neurodegeneration in vivo in C. elegans.

This dataset includes data files recording worm length after chemical exposure; neuronal damage scoring after chemical exposure; dopaminergic redox and bioenergetics states after chemical exposure; MATLAB scripts for quantification of dopaminergic redox state and quantification of dopaminergic bioenergetics state; and a data dictionary.