Description of methods used for collection/generation of data: Using the TaqMan (R) OpenArray (R) Rodent MicroRNA Panel (ThermoFisher Cat No. 4461105) high-throughput RT-qPCR was performed to measure the expression of microRNAs between experimental groups using the methods recommended by the supplier.
Experimental conditions: 5 female rats per group. 3 groups. Using 2 osmotic mini pumps for subcutaneous drug delivery rats were either treated with the COMT enzyme inhibitor OR486 or vehicle in one mini pump and the beta-2 and beta-3 adrenergic receptor inhibitors ICI-118551 and SR 59230A or respective vehicles. The groups represented in the data set are: "vv" = Vehicle/Vehicle "orv" = OR486/Vehicle and "oris" = OR486/ICI-118551 and SR 59230A. Whole blood from rats were collected after 14 days of treatment then centrifuged to isolate plasma. RNA was isolated from plasma using a RNA isolation from plasma kit (ThermoFisher) before cDNA synthesis. Synthesized cDNA was then used for the standard OpenArray protocol.
Publication under review will list more detailed methods for RNA isolation.
Experimental conditions: 5 female rats per group. 3 groups. Using 2 osmotic mini pumps for subcutaneous drug delivery rats were either treated with the COMT enzyme inhibitor OR486 or vehicle in one mini pump and the beta-2 and beta-3 adrenergic receptor inhibitors ICI-118551 and SR 59230A or respective vehicles. The groups represented in the data set are: "vv" = Vehicle/Vehicle "orv" = OR486/Vehicle and "oris" = OR486/ICI-118551 and SR 59230A. Whole blood from rats were collected after 14 days of treatment then centrifuged to isolate plasma. RNA was isolated from plasma using a RNA isolation from plasma kit (ThermoFisher) before cDNA synthesis. Synthesized cDNA was then used for the standard OpenArray protocol.
Publication under review will list more detailed methods for RNA isolation.