Glioblastoma (GBM) remains an untreatable malignant tumor with poor patient outcomes characterized by palisading necrosis and microvascular proliferation. While single-cell technology made it possible to characterize different lineage of glioma cells into neural progenitor-like (NPC-like) oligodendrocyte-progenitor-like (OPC-like) astrocyte-like (AC-like) and mesenchymal like (MES-like) states it does not capture the spatial localization of these tumor cell states. Spatial transcriptomics empowers the study of the spatial organization of different cell types and tumor cell states and allows for the selection of regions of interest to investigate region-specific and cell-type-specific pathways. We obtained paired 10x Chromium single-nuclei RNA-sequencing (snRNA-seq) and 10x Visium spatial transcriptomics data from three GBM patients to interrogate the GBM microenvironment. Here we have deposited the high resolution hematoxylin- and eosin-stained (H&E) images associated with the spatial transcriptomic data. The spatial transcriptomic data is restricted and must be housed elsewhere.
