This readme file was generated on 2025-02-07 by Ke Zoe Shan

-------------------
GENERAL INFORMATION
-------------------

Title of Dataset: Data from: PIEZO1 drives trophoblast fusion and placental development

Author Contact Information (Name, Institution, Email, ORCID)

	Principal Investigator: Huanghe Yang
	Institution: Duke University
	Email: Huanghe.yang@duke.edu
	ORCID: 0000-0001-9521-9328

	Associate or Co-investigator:
	Institution: Ke Zoe Shan
	Email: ke.shan@duke.edu
	ORCID: 0000-0002-2451-5813

	Alternate Contact(s): Ke Zoe Shan

*Date of data collection: 20210101-20250101

*Geographic location of data collection: Durham, North Carolina, USA 
 
*Funding and grant numbers (if applicable): R35GM153196 and DP2GM126898

--------------------
DATA & FILE OVERVIEW
--------------------

File list (filenames, directory structure (for zipped files) and brief description of all data files):

1-CD31 staining (6 files, TIF format)
	-Wildtype mouse placentas at E13.0 (3 files): 
		1006 WT#8 3, 
		1006 WT#9, 
		1006 WT#10 
	-Piezo1 conditional knockout (cKO) placentas at E13.0 (3 files):
		1006 cKO#5, 
		1006 cKO#6, 
		1006 cKO#7
 
2-H&E staining (10 files, PNG format)
	-Mouse placentas of E11.5 - wildtype (2 files):
		0920 WT#7-Stitching-14, 
		0921 WT#7-Stitching-10
	-Mouse placentas of E11.5 - Piezo1 cKO (2 files):
		0920 cKO#1-Stitching-15, 
		0921 cKO#6, 
	-Mouse placentas of E13.0 - wildtype (3 files):
		1006 WT#8-Stitching-01, 
		1006 WT#9-Stitching-04, 
		1006 WT#10-Stitching-06 
	-Mouse placentas of E13.0- Piezo1 cKO (3 files):
		1006 cKO#5-Stitching-03, 
		1006 cKO#6-Stitching-05, 
		1006 cKO#7-Stitching-07
 
3-MCT staining (two subfolders)
(1) cKO MCT: MCT1 and MCT4 staining of E13.0 mouse placentas of wildtype and Piezo1 cKO mice 
	     (22 files: 11 files in LSM format and their corresponding files in JPG format)
	-cKO:
		0224 cKO Image 1_Stitch (MCT1:green; MCT4:red), 
		0608 cKO Image 12_Stitch (MCT1:green; MCT4:red), 
		0608 cKO Image 31_Stitch (MCT1:green; MCT4:red), 
		1006 cKO#5 tile_Stitch (MCT1:green; MCT4:red), 
		1006 cKO#6 tile_Stitch (MCT1:green; MCT4:red), 
		1027 cKO Image 9_Stitch (MCT1:RED; MCT4:GREEN)
	-Wildtype:
		0224 WT Image 5_Stitch (MCT1:green; MCT4:red), 
		0608 WT Image 23_Stitch (MCT1:green; MCT4:red), 
		1006 WT#8 tile_Stitch (MCT1:green; MCT4:red), 
		1006 WT#9 tile_Stitch (MCT1:green; MCT4:red), 
		1027 WT Image 16_Stitch (MCT1:RED; MCT4:GREEN)

	-All the files are maximum intensity projection.
 
(2) Piezo1-TdTomato MCT: MCT1 (green), MCT4 (red) and PIEZO1-tdTomato (magenta) staining of E13.0 placentas from Piezo1-TdTomato mice 
		         (7 files in czi format)
		Airyscan Processing-01, 
		Airyscan Processing-02, 
		Airyscan Processing-03, 
		Airyscan Processing-04, 
		Airyscan Processing-05, 
		Airyscan Processing-06, 
		Airyscan Processing-07. 

	-All the files are maximum intensity projection.

--------------------------
METHODOLOGICAL INFORMATION
--------------------------

Description of methods used for collection/generation of data: 

For CD31 and H&E staining, microscopic imaging of the samples was taken at 20X by using an Olympus microscope (BX63l, Olympus, Center Valley, PA) with cellSens Dimension software.

For Immunofluorescence, microscopic imaging of the samples was taken with a Zeiss 780 inverted confocal microscope.

All image files can be opened in ImageJ.

-------------------------
USE and ACCESS INFORMATION
--------------------------

Data License: CC0

Other Rights Information: 

To cite the data: Yang, H., Zhang, Y., Shan, K., & Liang, P. (2025). Data from: PIEZO1 drives trophoblast fusion and placental development.Duke Research Data Repository. https://doi.org/10.7924/r4fj2qc4x