------------------- GENERAL INFORMATION ------------------- Title of Dataset: Data from: Irgm proteins attenuate inflammatory disease in mouse models of genital Chlamydia infection PMID- 38501887 Author Contact Information (Name, Institution, Email, ORCID) Principal Investigator: Jörn Coers Institution: Duke University Medical School Email: Jorn.coers@duke.edu ORCID: 0000-0001-8707-4608 Alternate Contact(s): *Date of data collection (single date, range, approximate date): years 2019 - 2023 *Geographic location of data collection (if applicable): Duke University Durham, NC USA *Funding and grant numbers (if applicable): R01 AI103197/AI/NIAID NIH HHS/ R01 AI148243/AI/NIAID NIH HHS/ -------------------- DATA & FILE OVERVIEW -------------------- File list (filenames, directory structure (for zipped files) and brief description of all data files): Figure 1.xlsx Fig 1 A defect in cell-autonomous immunity correlates with a transient increase in C. trachomatis burden in the female genital tract of pan-Irgm−/− mice. Data represent one experiment (n = 4 mice per genotype). Statistical significance was determined using t-test * p<0.05, *** p<0.0005, **** p<0.00005, ns not significant Figure 2.xlsx Fig 2 Pan-Irgm−/− mice display increased inflammation in genital C. trachomatis infection.Genital tracts were fixed, sectioned, and H&E stained. Mildly inflamed wildtype uterine tissue and severely inflamed and edematous pan-Irgm-/- uterus; wildtype ovary and oviduct with no evidence of inflammation and pan-Irgm-/- ovary, oviduct, and mesosalpinx each displaying severe inflammation . The magnitude of acute and chronic inflammation (C), edema (D), and dilation (E) were graded by a veterinary pathologist (n = 7 mice; each data point represents one uterine horn of an infected mouse). Statistical significance was determined using Mann-Whitney test (C-E); * p<0.05, **** p<0.00005 Figure 3.xlsx Fig 3 Pan-Irgm−/− mice display granulomatous inflammation in genital C. trachomatis infection. Quantification of number of uterine horns displaying granulomatous lesions (n = 7 mice). Data represents one experiment that is representative of multiple replicates. Statistical significance was calculated using chi square test; * p<0.05 Figure 4.xlsx Fig 4 Host resistance to C. muridarum remains unchanged in the absence of all three murine Irgm paralogs.Mice were infected transcervically with 2.5x105 C. muridarum EBs and genital tracts were harvested at the indicated timepoints. DNA was extracted and bacterial burden was quantified using qPCR. Data represent pooled data from one experiment including all time points plus one additional experiment for the 1 dpi time point. 4 hpi n = 4 mice; 1 dpi n = 9; 3 dpi n = 4; 6 dpi n = 4; 25 dpi wildtype n = 3, pan-Irgm-/- n = 4; 45 dpi n = 5. Statistical significance was determined using t-test; ns not significant Figure 5.xlsx Fig 5 Increased inflammation in pan-Irgm−/− at 6 dpi in genital C. muridarum infection. Mice were infected transcervically with 2.5 × 105 C. muridarum EBs and harvested at 6 dpi. Mice were infected transcervically with 2.5x105 C. muridarum EBs and harvested at 6 dpi. Genital tracts were fixed, sectioned, and H&E stained. The magnitude of acute and chronic inflammation (B) and dilation (C) were graded by a veterinary pathologist. Data represents one experiment that is representative of multiple replicates. Wildtype n = 6 mice, pan-Irgm-/- n = 10; each data point represents one uterine horn of an infected mouse. Statistical significance was determined using Mann-Whitney test; * p<0.05, ns = not significant Figure 6.xlsx Fig 6 Increased pathology in pan-Irgm−/− mice at later timepoints in genital C. muridarum infection. Mice were infected transcervically with 2.5 × 105 C. muridarum EBs and harvested at 25 dpi or 45 dpi. Mice were infected transcervically with 2.5x105 C. muridarum EBs and harvested at 25 dpi or 45 dpi. Genital tracts were fixed, sectioned, and H&E stained. The magnitude of acute, chronic, and total acute + chronic inflammation at 45 dpi were graded by a veterinary pathologist (wildtype n = 7 mice, pan-Irgm-/- n = 8). Data represent one experiment that is representative of multiple replicates. Statistical significance was determined using Mann-Whitney tests * p<0.05, ** p<0.005, ns not significant. $ - uterine horn not captured on histology slide and therefore not scored for pathology Figure 7.xlsx Fig 7 Increased inflammatory pathology in Irgm- and Rag1-deficient uteri in genital C. muridarum infection at 45 dpi. Mice were infected transcervically with 2.5 × 105 C. muridarum EBs. Genital tracts were harvested at 45 dpi, fixed, sectioned, H&E stained, and graded by a veterinary pathologist. Mice were infected transcervically with 2.5x105 C. muridarum EBs. Genital tracts were harvested at 45 dpi, fixed, sectioned, H&E stained, and graded by a veterinary pathologist. A combined uterine pathology index consisting of the sum of acute inflammation, chronic inflammation, dilation, glandular pathology, luminal exudates, and granulomatous inflammation was calculated. Each data point represents combined pathology scores from one entire reproductive tract from an infected mouse. Figure represents pooled data from two independent experiments (wildtype n = 7 mice, Irgm2-/- n = 5, Irgm3-/- n = 12, Irgm1/m3-/- n = 12, pan-Irgm-/- n = 10, Rag1/pan-Irgm-/- n = 9). Statistical significance was determined using Mann-Whitney tests; * p<0.05, ** p<0.005, *** p<0.0005, **** p<0.00005 Supplement.xlsx supplemental material Figure S1. Increased inflammation, tissue distortion, and pathology in Irgm- and Rag1-deficient C. muridarum infected mice. Mice were infected transcervically with 2.5x105 C. muridarum EBs. Genital tracts were harvested at 45 dpi, fixed, sectioned, H&E stained, and graded by a veterinary pathologist. Graphs represent the magnitude of acute inflammation, chronic inflammation, combined acute and chronic inflammation, and tissue dilation as defined by standard scoring criteria in the uteri (A), oviducts (B), and ovaries (C) of infected mice. Graphs represent pooled data from two independent experiments (wildtype n = 7 mice, Irgm2-/- n = 5, Irgm3-/- n = 12, Irgm1/m3-/- n = 12, pan-Irgm-/- n = 10, Rag1/pan-Irgm-/- n = 9; each data point represents one uterine horn of an infected mouse). Statistical significance was determined using Mann-Whitney tests; * p<0.05, ** p<0.005, *** p<0.0005, **** p<0.00005; all comparisons not indicated as significant were not statistically significant *Relationship between files, if important for context: Each file corresponds to the data shown in the identically named Figure in PMID- 38501887 *If data was derived from another source, list source citation: N/A -------------------------- METHODOLOGICAL INFORMATION -------------------------- Description of methods used for collection/generation of data: as described in PMID- 38501887 *Software- or Instrument-specific information needed to interpret the data, including software version numbers, packages or other dependencies: N/A *Standards and calibration information, if appropriate: N/A *Environmental/experimental conditions: as described in PMID- 38501887 -------------------------- DATA-SPECIFIC INFORMATION -------------------------- Variable/field list Define each including spelling out abbreviations as described in PMID- 38501887 Value/attribute list Include units of measure, codes or symbols used as described in PMID- 38501887 Missing data treatments (null, -99, na, etc.) N/A ------------------------- USE and ACCESS INFORMATION -------------------------- Data License/ Other Rights Information: For this Work published in mBio (including but not limited to text, figures, tables, artwork, abstracts, cover images, summaries, and supplemental material submitted with the Work), the Author has granted to the American Society for Microbiology (ASM) the full term of copyright and any extensions thereto the irrevocable, nonexclusive, worldwide license on a commercial and noncommercial basis (a) to publish, reproduce, distribute, transmit, display, and store the Work in all forms, now known or later developed; (b) to translate the Work into other languages, create adaptations, summaries, or extracts of the Work or other derivative works based on the Work and exercise all of the rights set forth in (a) above in such translations, adaptations, summaries, extracts, and derivative works; (c) to sublicense to others to do any or all of the above; and (d) to take any and all action to protect the copyright in the Work on behalf of the Author. It is understood that the Author will receive no royalty or other monetary compensation. To cite the data: mBio. 2024 Apr 10;15(4):e0030324. doi: 10.1128/mbio.00303-24. Epub 2024 Mar 19.