This readme file was generated on [2023-10-23] by [Arpine Sokratian]

GENERAL INFORMATION

Title of Dataset: Data from: Gut mucosal cells transfer α-synuclein to the vagus nerve 

Author/Principal Investigator Information
Name: Rodger Liddle
ORCID: 0000-0002-5498-4151
Institution: Duke University Medical Center
Address: 1033A Genome Science Research Building -1; 905 LaSalle Street
Email: rodger.liddle@duke.edu

Author/Associate or Co-investigator Information
Name: Andrew B. West
ORCID: 0000-0002-3034-4061
Institution: MRSBIII Duke University
Address: 3 Genome Ct., Durham, NC, 27710
Email: Andrew.West@Duke.edu 

Author/Alternate Contact Information
Name: Arpine Sokratian
ORCID:0000-0001-6654-5773
Institution: MRSBIII Duke Universit
Address: 3 Genome Ct., Durham, NC, 27710
Email: arpine.sokratian@duke.edu 

Date of data collection: 2021-01-15 - 2023-06-07> 

Geographic location of data collection: Durham, North Carolina



SHARING/ACCESS INFORMATION

Licenses/restrictions placed on the data: N/A

Links to publications that cite or use the data: in press, no DOI yet

Links to other publicly accessible locations of the data: Preprint doi: 10.1101/2023.08.14.553305. 

Links/relationships to ancillary data sets: N/A

Was data derived from another source? No
If yes, list source(s): 

Recommended citation for this dataset: : Chandra, R. Sokratian, A., Chavez K. R., King, S., Swain S. M., Snyder, J. C.,
West A. B., Liddle, R. A. (2023). Data from: Gut mucosal cells transfer α-synuclein to the vagus nerve. Duke Research Data Repository.
https://doi.org/10.7924/r4542x03c


DATA & FILE OVERVIEW

File List:  Raw data_rev_ver_final.xlsx - raw data used to build figure sets

There are five tabs in this file. Each tab refers to a corresponding figure.
Figure 1: Alpha-synuclein expression and seeding activity in SNCAA53T mice (Seeding amplification assay and ELISA raw data)
Figure 3: Conditional human α-synuclein expression induces α-synuclein seeding activity in gut organoids (Seeding amplification assay raw data)
Figure 4: Conditional human α-synuclein expression in gut mucosal cells produces in α-synuclein seeding activity in nodose ganglia (ELISA and seeding amplification assay raw data)
Figure 5: Vagotomy spares the nodose ganglia from α-synuclein seeding activity and prevents spread to the hindbrain (ELISA and seeding amplification assay raw data)
Sup.fig.1: Spike-in recombinant fibril seeds demonstrates sensitivity of RT-QuIC assays in protein lysates from Snca-/- nodose ganglia lysates (standard seeding amplification assay raw data)


METHODOLOGICAL INFORMATION

Description of methods used for collection/generation of data: A full description of the analysis methods can be found in the
preprint version of the manuscript doi: 10.1101/2023.08.14.553305

People involved with sample collection, processing, analysis and/or submission: 
Rashmi Chandra, Arpine Sokratian, Katherine R Chavez, Stephanie King, Sandip M Swain, Joshua C Snyder, Andrew B West, Rodger A Liddle