Readme GENERAL INFORMATION Title of Dataset: Nested circuits mediate the decision to vocalize Author/Principal Investigator Information Name: Richard Mooney ORCID: 0000-0002-3308-1367 Institution: Duke University Email: mooney@neuro.duke.edu SHARING/ACCESS INFORMATION Licenses/restrictions placed on the data: N/A Links to publications that cite or use the data: Shuyun Xiao, Valerie Michael, Richard Mooney (2023) Nested circuits mediate the decision to vocalize eLife 12:e85547. https://doi.org/10.7554/eLife.85547 Links to other publicly accessible locations of the data: https://elifesciences.org/articles/85547 Recommended citation for this dataset: Shuyun X., Michael, V., Mooney, R. (2023) Data from: Nested circuits mediate the decision to vocalize. Duke Research Data Repository. http://doi.org/10.7924/r4x35002b DATA & FILE OVERVIEW File List: There are 3 types of data in the repository: (1) confocal microscope images, (2) audio and video files from the mice used in this study and (3) slice electrophysiology data. All other data analyzed in this study are included in the manuscript and supporting files. : contains raw USV recordings with laser stimulations and videos related to Figure 2. In this experiment, ChR2 was expressed in PAG-projecting amygdala neurons in female mice. Results show that activating these neurons suppresses female USV production. Subfolders correspond with individual animals. : contains histology from this study. Confocal images related to transsynaptic rabies tracing shown in Figure 4 are included in this folder. Subfolders correspond with individual animals. : contains raw USV recordings with laser stimulations and videos related to Figure 3. In this experiment, ChR2 was expressed in Esr1+ amygdala neurons in male and female mice. Results show that activating these Esr1+ amygdala neurons suppresses vocalizations in both male and female mice. Subfolders correspond with individual animals. : contains photometry recordings, videos and audio files related to Figure 1, Figure 1-figure supplement 1, Figure 1-figure supplement 2, and Figure 5. Subfolders correspond with individual animals. : contains raw USV recordings with laser stimulations and videos related to Figure 6. In this experiment, ChR2 was expressed in amygdala-projecting POA neurons in male mice. Results show that activating these neurons is sufficient to promote USV production. : contains raw slice electrophysiology data related to Figure 6. : contains raw USV recordings and videos related to Figure 1 and Figure 2.