Data from: Cannabinoid exposure and altered DNA methylation in rat and human sperm

• This dataset contains the raw sequencing data files for reduced representation bisulfite sequencing. Abstract: Little is known about the reproductive effects of paternal cannabis exposure. We evaluated associations between cannabis or tetrahydrocannabinol (THC) exposure and altered DNA methylation in sperm from humans and rats, respectively. DNA methylation, measured by reduced representation bisulfite sequencing, differed in the sperm of human users from non-users by at least 10% at 3,979 CpG sites. Pathway analyses indicated Hippo Signaling and Pathways in Cancer as enriched with altered genes (Bonferroni p < 0.02). These same two pathways were also enriched with genes having altered methylation in sperm from THC-exposed versus vehicle-exposed rats (p < 0.01). Data validity is supported by significant correlations between THC exposure levels in humans and methylation for 177 genes, and substantial overlap in THC target genes in rat sperm (this study) and genes previously reported as having altered methylation in the brain of rat offspring born to parents both exposed to THC during adolescence. In humans, cannabis use was also associated with significantly lower sperm concentration. Findings point to possible pre-conception paternal reproductive risks associated with cannabis use. ... [Read More]

Total Size

49 files (177 GB)

Data Citation

• Murphy, S. K., Itchon-Ramos, N., Visco, Z., Huang, Z., Grenier, C., Schrott, R., Acharya, K., Boudreau, M.-H., Price, T. M., Raburn, D. J., Corcoran, D. L., Lucas, J. E., Mitchell, J. T., McClernon, J., Cauley, M., Hall, B. J., Levin, E. D., & Kollins, S. H. (2020). Data from: Cannabinoid exposure and altered DNA methylation in rat and human sperm. Duke Research Data Repository. https://doi.org/10.7924/r4v122j79

Creator

• Raburn, Douglas J.
• Levin, Edward D.
• Mitchell, John T.
• Kollins, Scott H.
• Acharya, Kelly
• Boudreau, Marie-Helene
• Grenier, Carole
• Itchon-Ramos, Nilda
• Visco, Zachary
• Hall, Brandon J.
• McClernon, Joseph
• Lucas, Joseph E.
• Price, Thomas M.
• Cauley, Marty
• Corcoran, David L.
• Schrott, Rose
• Murphy, Susan K.
• Huang, Zhiqing

DOI

• 10.7924/r4v122j79

Subject

• DNA Methylation
• Reduced Representation Bisulfite Sequencing (RRBS) DNA Methylation
• Cannabis
• Spermatozoa

Publication Date

• August 25, 2020

ARK

• ark:/87924/r4v122j79

Publisher

• Duke Research Data Repository

Type

• Dataset

Format

• FASTQ

Related Materials

• Murphy, S.K., Itchon-Ramos, N., Visco, Z., Huang, Z., Grenier, C., Schrott, R., Acharya, K., Boudreau, M.-H., Price, T.M., Raburn, D.J., Corcoran, D.L., Lucas, J.E., Mitchell, J.T., McClernon, J., Cauley, M., Hall, B.J., Levin, E.D., & Kollins, S.H. (2018). Cannabinoid exposure and altered DNA methylation in rat and human sperm. Epigenetics, 13(12), 1208-1221.  https://doi.org/10.1080/15592294.2018.1554521
• Schrott, R., Acharya, K., Itchon-Ramos, N., Hawkey, A.B., Pippen, E., Mitchell, J.T., Kollins, S.H., Levin, E.D., & Murphy, S.K. (2020). Cannabis use is associated with potentially heritable widespread changes in autism candidate gene DLGAP2 DNA methylation in sperm. Epigenetics, 15(1-2), 161-173.  https://doi.org/10.1080/15592294.2019.1656158

Funding Agency

• The John Templeton Foundation

Grant Number

• Grant 60564

Contact

• Susan K. Murphy: susan.murphy@duke.edu, 919-681-3423, ORCID: 0000-0001-8298-7272

License

• Creative Commons CC0 1.0 Universal

Title

• Data from: Cannabinoid exposure and altered DNA methylation in rat and human sperm

License

• http://creativecommons.org/publicdomain/zero/1.0/

Repository

• Duke Research Data Repository

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	RRBS Human Information.xlsx	2020-08-25	Public	Download
	zr1706_1_s1_R1.fastq	2020-08-25	Public	Get file from Globus
	zr1706_1_s1_R2.fastq	2020-08-25	Public	Get file from Globus
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	zr1706_3_s1_R1.fastq	2020-08-25	Public	Get file from Globus
	zr1706_3_s1_R2.fastq	2020-08-25	Public	Get file from Globus
	zr1706_4_s1_R1.fastq	2020-08-25	Public	Get file from Globus
	zr1706_4_s1_R2.fastq	2020-08-25	Public	Get file from Globus
	zr1706_5_s1_R1.fastq	2020-08-25	Public	Get file from Globus