Data from: Cannabinoid exposure and altered DNA methylation in rat and human sperm

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  • This dataset contains the raw sequencing data files for reduced representation bisulfite sequencing. Abstract: Little is known about the reproductive effects of paternal cannabis exposure. We evaluated associations between cannabis or tetrahydrocannabinol (THC) exposure and altered DNA methylation in sperm from humans and rats, respectively. DNA methylation, measured by reduced representation bisulfite sequencing, differed in the sperm of human users from non-users by at least 10% at 3,979 CpG sites. Pathway analyses indicated Hippo Signaling and Pathways in Cancer as enriched with altered genes (Bonferroni p < 0.02). These same two pathways were also enriched with genes having altered methylation in sperm from THC-exposed versus vehicle-exposed rats (p < 0.01). Data validity is supported by significant correlations between THC exposure levels in humans and methylation for 177 genes, and substantial overlap in THC target genes in rat sperm (this study) and genes previously reported as having altered methylation in the brain of rat offspring born to parents both exposed to THC during adolescence. In humans, cannabis use was also associated with significantly lower sperm concentration. Findings point to possible pre-conception paternal reproductive risks associated with cannabis use. ... [Read More]

Total Size
49 files (177 GB)
Data Citation
  • Murphy, S.K., Itchon-Ramos, N., Visco, Z., Huang, Z., Grenier, C., Schrott, R., Acharya, K., Boudreau, M.-H., Price, T.M., Raburn, D.J., Corcoran, D.L., Lucas, J.E., Mitchell, J.T., McClernon, J., Cauley, M., Hall, B.J., Levin, E.D., & Kollins, S.H. (2020). Data from: Cannabinoid exposure and altered DNA methylation in rat and human sperm. Duke Research Data Repository. https://doi.org/10.7924/r4v122j79
DOI
  • 10.7924/r4v122j79
Publication Date
ARK
  • ark:/87924/r4v122j79
Type
Format
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Funding Agency
  • The John Templeton Foundation
Grant Number
  • Grant 60564
Contact
Title
  • Data from: Cannabinoid exposure and altered DNA methylation in rat and human sperm

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