Data from: Gigapixel behavioral and neural activity imaging with a novel multi-camera array microscope

Public
Get Data from Globus
More About Globus

  • The dynamics of living organisms are organized across many spatial scales, yet existing, cost-effective imaging systems can measure only a subset of these scales at once. Here, we have created a scalable multi-camera array microscope (MCAM) that enables comprehensive high-resolution recording from multiple spatial scales simultaneously, ranging from cellular structures to large-group behavioral dynamics. By collecting data from up to 96 cameras, we computationally generate gigapixel-scale images and movies near cellular resolution and 5 um sensitivity over hundreds of square centimeters. This allows us to observe the behavior and fine anatomical features of numerous freely moving model organisms on multiple spatial scales, including larval zebrafish, fruit flies, nematodes, carpenter ants, and slime mold. The MCAM architecture allows stereoscopic tracking of the z-position of organisms using the overlapping field of view from adjacent cameras. Further, we demonstrate the ability to acquire dual color fluorescence video of multiple freely moving zebrafish, recording neural activity via ratiometric calcium imaging. Overall, the MCAM provides a powerful platform for investigating cellular and behavioral processes across a wide range of spatial scales, but without the bottlenecks imposed by single-camera image acquisition systems. ... [Read More]

Total Size
11 files (263 GB)
Data Citation
  • Thomson, E., Harfouche, M., Konda, P., Seitz, C., Kim, K., Cooke, C., Xu, S., Blazing, R., Chen, Y., Jacobs, W., Park, J., Dunn, T., Sharma, S., Horstmeyer, R., Naumann, E. (2022). Data from: Gigapixel behavioral and neural activity imaging with a novel multi-camera array microscope. Duke Research Data Repository. https://doi.org/10.7924/r4nv9kp8v
DOI
  • 10.7924/r4nv9kp8v
Publication Date
ARK
  • ark:/87924/r4nv9kp8v
Type
Format
Related Materials
  • Thomson, E. E., Harfouche, M., Kim, K., Konda, P. C., Seitz, C. W., Cooke, C., Xu, S., Jacobs, W. S., Blazing, R., Chen, Y., Sharma, S., Dunn, T. W., Park, J., Horstmeyer, R. W., & Naumann, E. A. (2022). Gigapixel imaging with a novel multi-camera array microscope. eLife, 11, e74988.  https://doi.org/10.7554/eLife.74988
Title
  • Data from: Gigapixel behavioral and neural activity imaging with a novel multi-camera array microscope
License
Repository
Thumbnail Title Date Uploaded Actions
file details 12 Drosophila adult bright-field imaging demonstration.zip 2022-02-10 Public
Get file from Globus

Home
This Dataset
Usage Stats