Data from: Ildr1 gene deletion protects against diet-induced obesity and hyperglycemia

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  • Objective
    Immunoglobulin-like Domain-Containing Receptor 1 (ILDR1) is expressed on nutrient sensing cholecystokinin-positive enteroendocrine cells of the gastrointestinal tract and it has the unique ability to induce fat-mediated CCK secretion. However, the role of ILDR1 in cholecystokinin-mediated regulation of satiety is unknown. In this study, we examined the effects of ILDR1 on food intake and metabolic activity using mice with genetically-deleted Ildr1.

    Methods
    The expression of ILDR1 in murine tissues and the measurement of adipocyte cell size were evaluated by light and fluorescence confocal microscopy. The effects of Ildr1 deletion on mouse metabolism were quantitated using CLAMS chambers and by targeted metabolomics assays of multiple tissues. Hormone levels were measured by ELISA. The effects of Ildr1 gene deletion on glucose and insulin levels were determined using in vivo oral glucose tolerance, meal tolerance, and insulin tolerance tests, as well as ex vivo islet perifusion.

    Results
    ILDR1 is expressed in a wide range of tissues. Analysis of metabolic data revealed that although Ildr1-/- mice consumed more food than wild-type littermates, they gained less weight on a high fat diet and exhibited increased metabolic activity. Adipocytes in Ildr1-/- mice were significantly smaller than in wild-type mice fed either low or high fat diets. ILDR1 was expressed in both alpha and beta cells of pancreatic islets. Based on oral glucose and mixed meal tolerance tests, Ildr1-/- mice were more effective at lowering post-prandial glucose levels, had improved insulin sensitivity, and glucose-regulated insulin secretion was enhanced in mice lacking ILDR1.

    Conclusion
    Ildr1 loss significantly modified metabolic activity in these mutant mice. While Ildr1 gene deletion increased high fat food intake, it reduced weight gain and improved glucose tolerance. These findings indicate that ILDR1 modulates metabolic responses to feeding in mice.     ... [Read More]

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18 files (597 MB)
Data Citation
  • Chandra, R., Aryal, D. K., Douros, J. D., Shahid, R., Davis, S. J., Campbell, J. E., Illkayeya, O., White, P. J., Rodriguez, R., Newgard, C. B., Wetsel, W. C., Liddle, R. A. (2022). Data from: Ildr1 gene deletion protects against diet-induced obesity and hyperglycemia. Duke Research Data Repository. https://doi.org/10.7924/r4m61nk31
DOI
  • 10.7924/r4m61nk31
Publication Date
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  • ark:/87924/r4m61nk31
Location
  • Durham, North Carolina, United States
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Related Materials
  • Chandra R, Aryal DK, Douros JD, Shahid R, Davis SJ, Campbell JE, Ilkayeya O, White PJ, Rodriguez R, Newgard CB, Wetsel WC, Liddle RA. Ildr1 gene deletion protects against diet-induced obesity and hyperglycemia. PLoS One. 2022 Jun 24;17(6):e0270329. doi: 10.1371/journal.pone.0270329. PMID: 35749484; PMCID: PMC9231709;  https://doi.org/10.1371/journal.pone.0270329
Funding Agency
  • Swarthmore College BRPC receives financial support from the Duke University School of Medicine and the Duke Cancer Institute Cancer Center Support Grant (NCI CCSG P30 CA014236)
  • David Baltimore/Broad Foundation summer research fellowship
  • National Institute of Diabetes and Digestive and Kidney Diseases
  • F32DK115031 https://researchtraining.nih.gov/programs/fellowships/f32
  • P30 National Institute of Diabetes and Digestive and Kidney Diseases
Grant Number
  • F32DK115031
  • DK064213
  • DK091946
  • P30 DK124723
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  • Data from: Ildr1 gene deletion protects against diet-induced obesity and hyperglycemia
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file details Chandra_Readme.txt 2022-05-05 Public Download
file details Bioassay CCK levels in CLAMS mice.pzf 2022-05-05 Public Download
file details Fig1_Raw image files.zip 2022-05-05 Public Download
file details Fig2_Fig4_Fig5_Prism and CSV export.zip 2022-05-05 Public Download
file details Fig3_Prism and CSV export.zip 2022-05-05 Public Download
file details Fig6_Prism and CSV export.zip 2022-05-05 Public Download
file details Fig7_Prism and CSV raw data.zip 2022-05-05 Public Download
file details Fig8_Prism and CSV export.zip 2022-05-05 Public Download
file details Fig9_Raw image files.zip 2022-05-05 Public Download
file details Fig10_Raw image files.zip 2022-05-05 Public Download

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