Data from: Effects of preconception exposure to rotenone on progeny depend on the degree of maternal toxicity

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  • Parental exposure to toxicants can affect progeny health, including when those exposures occur prior to conception. However, most published preconception studies have involved exposures that result in loading of pollutants to gametes, or toxic effects to parents which could indirectly affect germ cell or gamete health. Here, we took advantage of the biology of Caenorhabditis elegans to carry out a preconception study in which we minimized the potential for maternal loading of toxicants, and used an exposure paradigm that either did (high concentration) or did not (low concentration) significantly impact the health of the P0 generation. We hypothesized that preconception exposure to mitochondrial toxicants during germ cell and gamete development, at levels not causing P0 toxicity, would result in altered mitochondria and organismal health in offspring. In the P0 generation, the high rotenone concentration altered growth, mitochondrial respiration, gene expression, induction of the mitochondrial unfolded protein response, and susceptibility to dopaminergic neurodegeneration induced by a chemical “rechallenge” later in life. However, we observed minor or no effects in P0 at the low concentration. In high-exposure F1 offspring, we observed altered embryo size, larval developmental stage distribution, spare respiratory capacity, heat shock protein expression, and dopaminergic neurodegeneration after a secondary rotenone challenge. The only effects observed in the F1 offspring of the low exposure were a 1.7% decrease in egg size (size later in development was normal), and moderate evidence of a slightly increased sensitivity to heat shock protein expression and dopaminergic neurodegeneration caused by a secondary later-in-life rotenone exposure. ... [Read More]

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11 files (2.52 MB)
Data Citation
  • Bergemann, C. M., Mello, D. F., Chitrakar, R., Fisher, K., Bijwadia, S. R., Huayta, J., Ryde, I. T., Presman, R., Huang, Z., Herring, A., Murphy, S. K., Baugh, R., & Meyer, J. N. (2025). Data from: Effects of preconception exposure to rotenone on progeny depend on the degree of maternal toxicity. Duke Research Data Repository. https://doi.org/10.7924/r4c82jx2p
DOI
  • 10.7924/r4c82jx2p
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ARK
  • ark:/87924/r4c82jx2p
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Funding Agency
  • National Institutes of Health
Grant Number
  • R01ES028218
  • P42ES010356
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  • Data from: Effects of preconception exposure to rotenone on progeny depend on the degree of maternal toxicity
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file details OCR_seahorse_values.csv 2025-06-06 Public Download
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file details copy_number_values.csv 2025-06-06 Public Download
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file details developmental_stage.csv 2025-06-06 Public Download
file details egg_dimensions.csv 2025-06-06 Public Download
file details mitochondrial_UPR_values.csv 2025-06-06 Public Download
file details neurodegeneration_scores.csv 2025-06-06 Public Download
file details worm_age_estimation.csv 2025-06-06 Public Download

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