- The early stages of the virus-cell interaction have long evaded observation by existing microscopy methods due to the rapid diffusion of virions in the extracellular space and the large 3D cellular structures involved. Here we present an active-feedback single-particle tracking method with simultaneous volumetric imaging of the live cell environment to address this knowledge gap to present unprecedented detail to the extracellular phase of the infectious cycle. We report previously unobserved phenomena in the early stages of the virus-cell interaction, including skimming contact events at the millisecond timescale, orders of magnitude change in diffusion coefficient upon binding, and cylindrical and linear diffusion modes along cellular protrusions. ... [Read More]
- Total Size
- 55 files (50.8 GB)
- Data Citation
- Johnson, C., Exell, J., Lin, Y., Aguilar, J., Welsher, K. D. (2022). Data from: Capturing the start point of the virus-cell interaction with high-speed 3D single-particle tracking. Duke Research Data Repository. https://doi.org/10.7924/r4bp07h15.
- DOI
- 10.7924/r4bp07h15
- Publication Date
- August 29, 2022
- ARK
- ark:/87924/r4bp07h15
- Affiliation
- Publisher
- Type
- Format
- Related Materials
- Funding Agency
- NIH
- Grant Number
- R35GM124868
- Contact
- Welsher, Kevin D. https://orcid.org/0000-0002-3180-279X (919)660-1509 kevin.welsher@duke.edu
- Title
- Data from: Capturing the start point of the virus-cell interaction with high-speed 3D single-particle tracking
- Repository
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