Data from: Multimodal segmentation analysis of subcellular responses to hypo-osmotic stimulus using combined quantitative phase imaging and FRET-based calcium sensors.

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Here we seek to expand on previous work which combined Quantitative Phase Imaging (QPI) with a molecularly sensitive F√∂rster resonance energy transfer (FRET) construct by developing additional analysis techniques. This work seeks to further characterize the mechanotransduction of individual cells in response to stimulus through a novel QPI-guided cellular segmentation algorithm. The multimodal imaging instrument and analysis techniques are employed to examine cellular responses to hypo-osmotic stimulus by observing the calcium ion flux using a FRET based sensor coupled with a mapping of intracellular mass reorganization using QPI. The combined imaging modality enables a discrimination of cell response by localized region, revealing distinct behavior between regions and relative to a control group. ... [Read More]

Data Citation

Highland, R., Rancu, A., Price, H., Parker, S., Reynolds M., Hoffman, B., & Wax, A. (2024). Data from: Multimodal segmentation analysis of subcellular responses to hypo-osmotic stimulus using combined quantitative phase imaging and FRET-based calcium sensors. Duke Research Data Repository. http://doi.org/10.7924/r4tt4tp06

Creator: Wax, Adam
Rancu, Albert
Parker, Steven
Highland, Robert
Price, Hillel
Hoffman, Brent
Reynolds Meghan
DOI: 10.7924/r4tt4tp06
Subject: Calcium signaling
Microscopy
Applied optics
Mechanotransduction
Cell mechanics
Quantitative phase imaging
Forster resonance energy transfer (FRET)
Cell analysis
Fluorescence imaging
Dual-modality imaging
Publication Date: March 8, 2024
ARK: ark:/87924/r4tt4tp06
Affiliation: Fitzpatrick Institute of Photonics
Pratt School of Engineering
Publisher: Duke Research Data Repository
Type: Dataset
Related Materials: Preprint: https://doi.org/10.1101/2023.05.05.539565
Funding Agency: United States Army Research Office
Grant Number: W911NF1910306
Contact: Robert Highland: robert.highland@duke.edu, https://orcid.org/0009-0007-3468-3224
License: Creative Commons CC0 1.0 Universal
Title: Data from: Multimodal segmentation analysis of subcellular responses to hypo-osmotic stimulus using combined quantitative phase imaging and FRET-based calcium sensors.
License: http://creativecommons.org/publicdomain/zero/1.0/
Repository: Duke Research Data Repository

Title	Date Uploaded	Visibility	Actions
Control Imaging Data.zip	2024-03-08	Public	Get file from Globus
HypoOsm Imaging Data.zip	2024-03-08	Public	Get file from Globus
Layer Correlation Data (ER, DAPI, QPI).zip	2024-03-08	Public	Get file from Globus
README_HIGHLAND_QPI-FRET.txt	2024-03-08	Public	Download
Repository Minimum Data Set.pdf	2024-03-08	Public	Download

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