- Transformed E. coli were grown overnight at 37 °C on Luria Bertani Broth (Lennox) (LB) agar plates containing the appropriate antibiotics (50 μg/mL kanamycin and/or 100 μg/mL ampicillin). A single colony was used to inoculate 5 mL of liquid LB with the appropriate antibiotics and grown at 37 °C and 200 rpm for 20 h. Cells were harvested by centrifugation (4000 rcf, 5 min, rt), resuspended in 0.5 mL of ultrapure water, and DNA was isolated using Zymo Research Zyppy Plasmid Miniprep Kit. Purified DNA was sent to plasmidsaurus for whole-plasmid Oxford Nanopore sequencing.
.dna files of plasmid maps can be opened with SnapGene Viewer, a ... [Read More]
- Total Size
- 10 files (574 KB)
- Data Citation
- Duda, A. M., Ma, H. R., Villalobos, C. A., Kuhn, S. A., Angle, S. S., He, K., Jackson, A. C., Suh, C. M., Puccio, E. A., Anderson, D. J., Fowler, V. G., You, L. & Franz, K. J. (2025). Data from: An engineered prodrug selectively suppresses β-lactam resistant bacteria in a mixed microbial setting. Duke Research Data Repository. https://doi.org/10.7924/r4vm4hd42
- Creator
- DOI
- 10.7924/r4vm4hd42
- Subject
- Publication Date
- May 30, 2025
- ARK
- ark:/87924/r4vm4hd42
- Affiliation
- Publisher
- Collection Dates
- June 13, 2023 to November 8, 2023
- Type
- Funding Agency
- National Institutes of Health
- Marcil-Monahan Scholars Initiative of Trinity College of Arts and Sciences, Duke University
- Grant Number
- T32 GM1333352
- Contact
- Katherine J. Franz, https://orcid.org/0000-0002-9015-0998, katherine.franz@duke.edu; Addison M. Duda, https://orcid.org/0000-0002-8116-4714, addison.duda@alumni.duke.edu
- Title
- Data from: An engineered prodrug selectively suppresses β-lactam resistant bacteria in a mixed microbial setting
- Repository
| Thumbnail | Title | Date Uploaded | Visibility | Actions |
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README.txt | 2025-05-30 | Download | |
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CMY-2.zip | 2025-05-30 | Download | |
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CTX-M-1.zip | 2025-05-30 | Download | |
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IMP-1.zip | 2025-05-30 | Download | |
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NDM-1.zip | 2025-05-30 | Download | |
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Null.zip | 2025-05-30 | Download | |
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OXA-1.zip | 2025-05-30 | Download | |
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TEM-1.zip | 2025-05-30 | Download | |
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VIM-2.zip | 2025-05-30 | Download | |
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mCherry.zip | 2025-05-30 | Download |