- Regulation of codon optimality is an increasingly appreciated layer of cell- and tissue-specific protein expression control. Here, we use codon-modified reporters to show that differentiation of Drosophila neural stem cells into neurons enables protein expression from rare-codon-enriched genes. From a candidate screen, we identify the cytoplasmic polyadenylation element binding (CPEB) protein Orb2 as a positive regulator of rare-codon-dependent mRNA stability in neurons. Using RNA sequencing, we reveal that Orb2-upregulated mRNAs in the brain with abundant Orb2 binding sites have a rare-codon bias. From these Orb2-regulated mRNAs, we demonstrate that rare-codon enrichment is important for mRNA stability and social behavior function of the metabotropic glutamate receptor (mGluR). ... [Read More]
- Total Size
- 8 files (75.2 MB)
- Data Citation
- Stewart, R. & Fox, D. (2025). Data from: Orb2 enables rare-codon-enriched mRNA expression during Drosophila neuron differentiation. Duke Research Data Repository. https://doi.org/10.7924/r4kh0v66f
- Creator
- DOI
- 10.7924/r4kh0v66f
- Subject
- Publication Date
- March 11, 2025
- ARK
- ark:/87924/r4kh0v66f
- Affiliation
- Publisher
- Type
- Related Materials
- Funding Agency
- National Institutes of Health
- Grant Number
- R01HD113543
- Contact
- Donald Fox: https://orcid.org/0000-0002-0436-179X
- Title
- Data from: Orb2 enables rare-codon-enriched mRNA expression during Drosophila neuron differentiation
- Repository
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README.txt | 2025-03-11 | Download | |
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figure 1.zip | 2025-03-11 | Download | |
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figure 2.zip | 2025-03-11 | Download | |
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figure 3.zip | 2025-03-11 | Download | |
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figure 4.zip | 2025-03-11 | Download | |
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figure 5.zip | 2025-03-11 | Download | |
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figure 6.zip | 2025-03-11 | Download | |
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supplemental.zip | 2025-03-11 | Download |