Data from: Differential sensitivity to interepitope spacing in mast cells and B cells enables design of hypoallergenic allergen vaccine immunogens

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Therapeutic allergen vaccine immunogens can trigger IgE-mediated mast cell activation, resulting in allergic reactions. Here, we report on a mode of hypoallergenic immunogen design that enables immunization against IgE-reactive peptide B cell epitopes by optimizing the distance between epitopes. Using DNA-based model immunogens, we show that mast cells and B cells exhibit idiosyncratic sensitivity to interepitope spacing, with mast cell activation being dampened by high interepitope spacing while B cells remain responsive to identical immunogen configurations. To exploit this finding, we construct hypoallergenic immunogens based on supramolecular peptide nanofibers with ultralow epitope density that, when used as an allergen vaccine, raise protective allergen-neutralizing IgG antibody responses. This study provides a proof-of-concept for a mode of hypoallergenic immunogen design based on nanoscale control of the distances between IgE-reactive epitopes, which may enable allergen vaccination against IgE-reactive epitope targets in the absence of allergic reactogenicity. ... [Read More]

Data Citation

Cossette, B. J., Shen, L., Bermudez, A., Freire Haddad, H., Shetty, S., Sylvers, J., Yuan, F., Ke, Y., & Collier, J. H. (2025). Data from: Differential sensitivity to interepitope spacing in mast cells and B cells enables design of hypoallergenic allergen vaccine immunogens. Duke Research Data Repository. https://doi.org/10.7924/r47d34n91

Creator: Cossette, Benjamin J.
Shetty, Shamitha
Sylvers, Justin
Collier, Joel H.
Ke, Yonggang
Shen, Luyao
Freire Haddad, Helena
Yuan, Fan
Bermudez, Andrés
DOI: 10.7924/r47d34n91
Subject: Nanostructures
Hypersensitivity
Immunoengineering
Publication Date: April 23, 2025
ARK: ark:/87924/r47d34n91
Affiliation: Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University Atlanta
Biomedical Engineering
Publisher: Duke Research Data Repository
Collection Dates: 2023-03-01-2024-03-01
Type: Dataset
Format: CSV
Related Materials: Cossette, B. J., Shen, L., Bermudez, A., Freire Haddad, H., Shetty, S., Sylvers, J., Yuan, F., Ke, Y., & Collier, J. H. (2025). Differential sensitivity to interepitope spacing in mast cells and B cells enables design of hypoallergenic allergen vaccine immunogens. ACS nano. https://doi.org/10.1021/acsnano.4c14507
Funding Agency: National Institutes of Health
Grant Number: 1R21AI164740
Contact: Joel Collier, joel.collier@duke.edu, https://orcid.org/0000-0003-3536-4827
License: Creative Commons CC0 1.0 Universal
Title: Data from: Differential sensitivity to interepitope spacing in mast cells and B cells enables design of hypoallergenic allergen vaccine immunogens
License: http://creativecommons.org/publicdomain/zero/1.0/
Repository: Duke Research Data Repository

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