Data from: SHR and SCR coordinate root patterning and growth early in the cell cycle

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  • Precise control of cell division is essential for proper patterning and growth during the development of multicellular organisms. Coordination of formative divisions that generate new tissue patterns with proliferative divisions that promote growth is poorly understood. SHORTROOT (SHR) and SCARECROW (SCR) are transcription factors (TFs) required for formative divisions in the stem cell niche of Arabidopsis roots. Here, we show that levels of SHR and SCR early in the cell cycle determine the orientation of the division plane, resulting in either formative or proliferative cell division. We employed 4D quantitative, long-term, and frequent (every 15 minutes for up to 48 hours) light sheet and confocal microscopy to probe the dynamics of SHR and SCR in tandem within single cells of living roots. Directly controlling their dynamics with a SHR induction system enabled us to challenge an existing bistable model of the SHR/SCR gene regulatory network and to identify key features that are essential for rescue of formative divisions in shr mutants. SHR and SCR kinetics do not align with the expected behavior of a bistable system, and only low transient levels, present early in the cell cycle, are required for formative divisions. These results reveal an uncharacterized mechanism by which developmental regulators directly coordinate patterning and growth. ... [Read More]

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80 files (566 GB)
Data Citation
  • Winter, C. M., Szekeley, P., Popov, V., Belcher, H., Carter, R., Jones, M., Fraser, S., Truong, T., & Benfey, P. (2023). Data from: SHR and SCR coordinate root patterning and growth early in the cell cycle. Duke Research Data Repository. https://doi.org/10.7924/r46q2571m
DOI
  • 10.7924/r46q2571m
Publication Date
ARK
  • ark:/87924/r46q2571m
Collection Dates
  • 2017 - 2023
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Related Materials
  • Winter, C. M., Szekely, P., Popov, V., Belcher, H., Carter, R., Jones, M., Fraser, S. E., Truong, T. V., & Benfey, P. N. (2024). SHR and SCR coordinate root patterning and growth early in the cell cycle. Nature, 626(7999), 611–616.  https://doi.org/10.1038/s41586-023-06971-z
Funding Agency
  • Translational Imaging Center, Bridge Institute, University of Southern California.
  • US National Institutes of Health
  • Howard Hughes Medical Institute
Grant Number
  • NRSA 5F32GM106690-02
  • MIRA 1R35GM131725
Title
  • Data from: SHR and SCR coordinate root patterning and growth early in the cell cycle
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Thumbnail Title Date Uploaded Visibility Actions
file details MetaData.csv 2023-10-26 Public Download
file details Cell_cycle_synchronization_timecourses 2023-11-01 Public
file details WT_timecourses 2023-11-01 Public
file details Light_Sheet_indSHR_SCRmKATE2_timecourses 2023-11-01 Public
file details PlaCCI_timecourses 2023-11-01 Public
file details Confocal_indSHR_timecourses 2024-01-11 Public

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