Data and Code from: T Cell-Depleted Cultured Pediatric Thymus Tissue as a Model for Some Aspects of Human Age-Related Thymus Involution

Public
Export Files
Get Data from Globus
More About Globus

  • This dataset contains the QuantiGene data and the code used to analyze it, as published in Hale, LP, et al. T Cell-Depleted Cultured Pediatric Thymus Tissue as a Model for Some Aspects of Human Age-Related Thymus Involution, GeroScience (accepted November 2020).

    Abstract: Human age-related thymus involution is characterized by loss of developing thymocytes and the thymic epithelial network that supports them, with replacement by adipose tissue. The mechanisms that drive these changes are difficult to study in vivo due to constant trafficking to and from the thymus. We hypothesized that the loss of thymocytes that occurs during human thymic organ cultures could model some aspects of thymus involution and begin to identify mechanisms that drive age related changes in the thymic microenvironment. Potential mechanistically important candidate molecules were initially identified by screening conditioned media from human thymus organ cultures using antibody microarrays. These candidates were further validated using cultured tissue extracts and conditioned media. Results were compared with gene expression studies from a panel of well-characterized (non-cultured) human thymus tissues from human donors aged 5 days to 78 years. L-selectin released into conditioned media was identified as a biomarker for the content of viable thymocytes within cultured thymus.

    Levels of the cheamokines CCL21 and CXCL12, likely produced by surviving thymic epithelial cells, increased markedly in conditioned media as thymocytes were lost during culture. Native non-cultured thymus from adults older than 18 years also showed a strong trend toward increased CCL21 expression, in conjunction with significant decreases in thymocyte-related mRNAs compared with thymus from subjects younger than 18 years. Together, these findings demonstrate that use of postnatal human thymus organ cultures can model some aspects of human age-related thymic involution.     ... [Read More]

Total Size
46 files (4.8 MB)
Data Citation
  • Hale, L., Cheatham, L., Macintyre, A., LaFleur, B., Sanders, B., Troy, J., Kurtzberg, J., & Sempowski, G. (2021). Data and Code from: T Cell-Depleted Cultured Pediatric Thymus Tissue as a Model for Some Aspects of Human Age-Related Thymus Involution. Duke Research Data Repository. https://doi.org/10.7924/r47d2xg50
DOI
  • 10.7924/r47d2xg50
Subject
Publication Date
ARK
  • ark:/87924/r47d2xg50
Language
Type
Format
Related Materials
  • Hale, L.P., Cheatham, L., Macintyre, A.N. et al. T cell–depleted cultured pediatric thymus tissue as a model for some aspects of human age-related thymus involution. GeroScience (2021).  https://doi.org/10.1007/s11357-020-00301-1
Funding Agency
  • Duke University Medical Center
  • Hartwell Foundation
  • Robertson Foundation
  • Enzyvant Therapeutics GmbH
  • National Institutes of Health
Grant Number
  • 5R01-AI047040
  • 5U01-HG007672
  • P01-AG052359
Title
  • Data and Code from: T Cell-Depleted Cultured Pediatric Thymus Tissue as a Model for Some Aspects of Human Age-Related Thymus Involution
License
Repository
Thumbnail Title Date Uploaded Visibility Actions
file details Hale_README.txt 2021-01-04 Public Download
file details RPubs - Code for GeoScience Manuscript.html 2021-01-04 Public Download
file details Code 2021-01-04 Public
file details Data 2021-01-04 Public
file details RPubs - Code for GeoScience Manuscript_files 2021-01-04 Public

Home
This Dataset
Usage Stats