Data and Code from: T Cell-Depleted Cultured Pediatric Thymus Tissue as a Model for Some Aspects of Human Age-Related Thymus Involution

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  • This dataset contains the QuantiGene data and the code used to analyze it, as published in Hale, LP, et al. T Cell-Depleted Cultured Pediatric Thymus Tissue as a Model for Some Aspects of Human Age-Related Thymus Involution, GeroScience (accepted November 2020).

    Abstract: Human age-related thymus involution is characterized by loss of developing thymocytes
    and the thymic epithelial network that supports them, with replacement by adipose tissue. The
    mechanisms that drive these changes are difficult to study in vivo due to constant trafficking to
    and from the thymus. We hypothesized that the loss of thymocytes that occurs during human
    thymic organ cultures could model some aspects of thymus involution and begin to identify
    mechanisms that drive age-related changes in the thymic microenvironment. Potential
    mechanistically important candidate molecules were initially identified by screening conditioned
    media from human thymus organ cultures using antibody microarrays. These candidates were
    further validated using cultured tissue extracts and conditioned media. Results were compared
    with gene expression studies from a panel of well-characterized (non-cultured) human thymus
    tissues from human donors aged 5 days to 78 years. L-selectin released into conditioned media
    was identified as a biomarker for the content of viable thymocytes within cultured thymus.
    Levels of the cheamokines CCL21 and CXCL12, likely produced by surviving thymic epithelial
    cells, increased markedly in conditioned media as thymocytes were lost during culture. Native
    non-cultured thymus from adults older than 18 years also showed a strong trend toward
    increased CCL21 expression, in conjunction with significant decreases in thymocyte-related
    mRNAs compared with thymus from subjects younger than 18 years. Together, these findings
    demonstrate that use of postnatal human thymus organ cultures can model some aspects of
    human age-related thymic involution.
    ... [Read More]

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46 files (4.8 MB)
Data Citation
  • Hale, L., Cheatham, L., Macintyre, A., LaFleur, B., Sanders, B., Troy, J., Kurtzberg, J., & Sempowski, G. (2021). Data and Code from: T Cell-Depleted Cultured Pediatric Thymus Tissue as a Model for Some Aspects of Human Age-Related Thymus Involution. Duke Research Data Repository. https://doi.org/10.7924/r47d2xg50
DOI
  • 10.7924/r47d2xg50
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  • ark:/87924/r47d2xg50
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Funding Agency
  • Duke University Medical Center
  • Robertson Foundation
  • Hartwell Foundation
  • National Institutes of Health
  • Enzyvant Therapeutics GmbH
Grant Number
  • 5U01-HG007672
  • P01-AG052359
  • 5R01-AI047040
Title
  • Data and Code from: T Cell-Depleted Cultured Pediatric Thymus Tissue as a Model for Some Aspects of Human Age-Related Thymus Involution

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