• This dataset contains the QuantiGene data and the code used to analyze it, as published in Hale, LP, et al. T Cell-Depleted Cultured Pediatric Thymus Tissue as a Model for Some Aspects of Human Age-Related Thymus Involution, GeroScience (accepted November 2020).
  
  Abstract: Human age-related thymus involution is characterized by loss of developing thymocytes
  and the thymic epithelial network that supports them, with replacement by adipose tissue. The
  mechanisms that drive these changes are difficult to study in vivo due to constant trafficking to
  and from the thymus. We hypothesized that the loss of thymocytes that occurs during human
  thymic organ cultures could model some aspects of thymus involution and begin to identify
  mechanisms that drive age-related changes in the thymic microenvironment. Potential
  mechanistically important candidate molecules were initially identified by screening conditioned
  media from human thymus organ cultures using antibody microarrays. These candidates were
  further validated using cultured tissue extracts and conditioned media. Results were compared
  with gene expression studies from a panel of well-characterized (non-cultured) human thymus
  tissues from human donors aged 5 days to 78 years. L-selectin released into conditioned media
  was identified as a biomarker for the content of viable thymocytes within cultured thymus.
  Levels of the cheamokines CCL21 and CXCL12, likely produced by surviving thymic epithelial
  cells, increased markedly in conditioned media as thymocytes were lost during culture. Native
  non-cultured thymus from adults older than 18 years also showed a strong trend toward
  increased CCL21 expression, in conjunction with significant decreases in thymocyte-related
  mRNAs compared with thymus from subjects younger than 18 years. Together, these findings
  demonstrate that use of postnatal human thymus organ cultures can model some aspects of
  human age-related thymic involution. ... [Read More]

Total Size

46 files (4.8 MB)

Data Citation

• Hale, L., Cheatham, L., Macintyre, A., LaFleur, B., Sanders, B., Troy, J., Kurtzberg, J., & Sempowski, G. (2021). Data and Code from: T Cell-Depleted Cultured Pediatric Thymus Tissue as a Model for Some Aspects of Human Age-Related Thymus Involution. Duke Research Data Repository. https://doi.org/10.7924/r47d2xg50

Creator

• Hale, Laura P
• Troy, Jesse
• Sanders, Brittany
• Cheatham, Lynn
• Macintyre, Andrew N
• Kurtzberg, Joanne
• LaFleur, Bonnie
• Sempowski, Gregory D

DOI

• 10.7924/r47d2xg50

Subject

• Gene expression

Publication Date

• January 4, 2021

ARK

• ark:/87924/r47d2xg50

Affiliation

• The BIO5 Institute, University of Arizona
• Pediatrics
• School of Medicine
• Pathology
• Marcus Center for Cellular Cures
• Duke Human Vaccine Institute

Publisher

• Duke Research Data Repository

Language

• English

Type

• Dataset

Format

• RMD
• HTML
• XLS

Funding Agency

• Enzyvant Therapeutics GmbH
• National Institutes of Health
• Duke University Medical Center
• Hartwell Foundation
• Robertson Foundation

Grant Number

• 5U01-HG007672
• P01-AG052359
• 5R01-AI047040

Contact

• Laura P. Hale, MD PhD: laura.hale@duke.edu; https://orcid.org/0000-0001-5707-0390

Rights

• Creative Commons CC0 1.0 Universal

Title

• Data and Code from: T Cell-Depleted Cultured Pediatric Thymus Tissue as a Model for Some Aspects of Human Age-Related Thymus Involution

Rights

• http://creativecommons.org/publicdomain/zero/1.0/

Repository

• Duke Research Data Repository

Items

Thumbnail	Title	Date Uploaded		Actions
	Hale_README.txt	2021-01-04	Public	Download
	RPubs - Code for GeoScience Manuscript.html	2021-01-04	Public	Download
	Code	2021-01-04	Public
	Data	2021-01-04	Public
	RPubs - Code for GeoScience Manuscript_files	2021-01-04	Public