Data from: In vivo pump-probe and multiphoton fluorescence microscopy of melanoma and pigmented lesions in a mouse model

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  • We demonstrate a multimodal approach that combines a pump--probe with confocal reflectance and multiphoton autofluorescence microscopy. Pump--probe microscopy has been proven to be of great value in analyzing thin tissue sections of pigmented lesions, as it produces molecular contrast which is inaccessible by other means. However, the higher optical intensity required to overcome scattering in thick tissue leads to higher-order nonlinearities in the optical response of melanin (e.g., two-photon pump and one-photon probe) that present additional challenges for interpreting the data. We show that analysis of pigment composition in vivo must carefully account for signal terms that are nonlinear with respect to the pump and probe intensities. We find that pump--probe imaging gives useful contrast for pigmented structures over a large range of spatial scales (100 μm to 1 cm), making it a potentially useful tool for tracking the progression of pigmented lesions without the need to introduce exogenous contrast agents. ... [Read More]

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39 files (34.8 GB)
Data Citation
  • Wilson, J. W., Degan, S., Gainey, C. S., Mitropoulos, T., Simpson, M. J., Zhang, J. Y., & Warren, W. S. (2019). Data from: In vivo pump-probe and multiphoton fluorescence microscopy of melanoma and pigmented lesions in a mouse model. Duke Digital Repository. https://doi.org/10.7924/r4cc0zp95
DOI
  • 10.7924/r4cc0zp95
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  • ark:/87924/r4cc0zp95
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Related Materials
  • Jesse W. Wilson, Simone Degan, Christina S. Gainey, Tanya Mitropoulos, Mary Jane Simpson, Jennifer Y. Zhang, Warren S. Warren, "Comparing in vivo pump–probe and multiphoton fluorescence microscopy of melanoma and pigmented lesions," J. Biomed. Opt. 20(5) 051012 (21 November 2014).  https://doi.org/10.1117/1.JBO.20.5.051012
Funding Agency
  • National Institutes of Health (U.S.)
Grant Number
  • R01-CA166555
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Title
  • Data from: In vivo pump-probe and multiphoton fluorescence microscopy of melanoma and pigmented lesions in a mouse model
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